Abstract: To study the anti-inflammatory effect and anti-inflammatory mechanism of Phillygenin,dexamethasone as a positive control,established a lipopolysaccharide (LPS)-induced RAW264.7 inflammation model,to detect the release of inflammatory factors and the expression of related proteins and mRNA,in order to improve the comprehensive understanding of the anti-inflammatory effect of Phillygenin and provide a strong scientific basis for the clinical development of Phillygenin.The NO production was determined by assaying nitrite in culture supernatants with the Griess reagent.The levels of TNF-α and IL-6 in culture media were measured with ELISA kits.Western blot assay was performed to illustrate the inhibitory effects of Phillygenin on iNOS and COX-2.RT-qPCR was detected for mRNA expression of iNOS and COX-2.Treatment with Phillygenin or Dexamethasone dose-dependently inhibited the production expression of NO,TNF-α and IL-6 in RAW264.7 macrophages.Western blot and RT-qPCR assay suggested that the mechanism of the anti-inflammatory effect was associated with the inhibition of iNOS and COX-2.In conclusion,Phillygenin exhibited obvious anti-inflammatory effect and its mechanism may be related to its regulation on inflammatory cytokines,and inhibition of the expression of iNOS and COX-2.

Key words: Phillygenin, dexamethasone, lipopolysaccharide, macrophage in mice, inflammatory cytokines