NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2019, Vol. 31 ›› Issue (8): 1418-1424.doi: 10.16333/j.1001-6880.2019.8.017

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Polysaccharide extraction and bioactivity test from Camellia japonica pollen and its rare earth elements and heavy metal elements content determination

ZHANG Duo1,YAN Rong-ling1*,LUO Zhi-wei1,HUANG Xiang-yu1,YAN Jiang-tao2,LIAO Yang1*   

  1. 1Key Laboratory of comprehensive utilization of dominant plant resources in southern Hunan,College of Chemistry and Bioengineering,Hunan University of Science and Engineering,Yongzhou 425199,China; 2Orthopedics hospital of Qinhuangdao,Qinhuangdao 066000,China
  • Online:2019-09-10 Published:2019-09-10

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Abstract: In order to obtain a better development and utilization of Camellia japonica Pollen,the content of rare earth elements (REE) and heavy metal elements (HME) in the pollen were determined by ICP-MS method,and the cellulose-microwave assisted extraction process for polysaccharide from the pollen and its anti-fatigue and immune improving activity were researched in the present research.Results showed that,there was an extremely low content of both REE and HME in C.japonica Pollen,total content of all 17 kinds REE was only 0.071 5 mg/kg.The element Lanthanum (La) showed the highest content (0.020 1 mg/kg),while Promethium (Pm) showed the least content (0.000 6 mg/kg),and 4 elements such as Holmium(Ho),Thulium (Tm),Lutetium (Lu),Scandium (Sc) were not detected.The content of HMEs such as Copper (Cu),Zinc (Zn),Cadmium (Cd) and Plumbum (Pb) were higher than that of REEs,the Cu and Zn content were more than 1.0 mg/kg while Chromium (Cr) and Hydrargyrum (Hg) were not detected.The optimum extraction process of polysaccharide from C.japonica Pollen were enzyme dosage 0.08 g/g,enzymatic hydrolysis time 80 min,microwave time 45 s,and microwave power 500 W.The maximum extraction rate was 2.69% under the optimal conditions.The blood lactic acid (BLA) concentration of intragastric administrated mice with extracted polysaccharide was less than that of control group (CK) after swimming,and the BLA concentration decayed more rapidly than CK in the followed resting stage after swiming;intragastric administrated with extracted polysaccharide improved the mice’ thymus and spleen index significantly (P<0.05) though the indexes did not fully recover to normal levels.Results indicate that the content of REE and HME in the C.japonica absolutely at a safe level,cellulase with microwave assisting could help us to obtain the polysaccharide from C.japonica pollen more quickly and effectively,and the extracted polysaccharide showed a good biological activity of antifatigue and immune improving.The research could provide some references for the comprehensive utilization of the pollen of C.japonica.

Key words: bioactivity, rare earth element, heavy metal element, polysaccharide, cellulase-microwave synergistic, Camellia japonica pollen

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