Abstract: In order to explore the influence of different dosage forms on the synthesis and accumulation of metabolites of Pseudostellariae Radix (PR),HPLC-Q-Exactive Orbitrap/MS method combined with multivariate statistical analysis was used to analyze the different chemical components in traditional PR Chinese medicine decoction pieces and PR formula particles.Based on the metabolomics technology of HPLC-Q-Exactive Orbitrap/MS,the components were identified by software database search.Principal component analysis (PCA),partial least square discriminant analysis (PLS-DA) and orthogonal least square discriminant analysis (OPLS-DA) were used to select and identify the different chemical components.Results showed that the traditional PR Chinese medicine decoction pieces and PR formula particles can significantly distinguish,98 differential chemical compositions were found in positive ion mode,while 52 differential chemical compositions were found in negative ion mode.The differential chemical compositions are mainly concentrated in flavonoids,organic acids,fatty acids,and amino acids compounds,and present different change rules,mainly involved in the isoquinoline alkaloid biosynthesis metabolic pathways.The study can provide basic information for revealing the influence law of different dosage forms on the synthesis and accumulation of metabolites in PR.

Key words: Pseudostellariae Radix, HPLC-Q-Exactive Orbitrap/MS, differential chemical compositions, traditional Chinese medicine decoction pieces, traditional Chinese medicine formula particles