To establish an HPLC method to generate the fingerprint of Platycodon grandiflorumfrom different regionsand combine with chemometricsto provide a reference for scientific evaluate quality.22 batches from 11 provinces were collected to establish the HPLC-ELSD fingerprint.Four peaks have been identified by chemical composition with in nine common peaks.All fingerprints were evaluated by luster analysis (CA),principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) methods.Combined with the climatic characteristics of the regions,the temperate monsoon climate was more suitable for the growth of the P. grandiflorum.It contained more chemical components and had better quality.The method was fast,accurate,highly sensitive,andrepeatable.Combined with chemometric analysis,the method could be used to classify the quality and provide a scientific basis of P. grandiflorum in different origins.