In order to make better use of the two local characteristic resources of Yongzhou,the composition of the Yongzhou mint essential oil was analyzed by GC-MS,the antimicrobial effect of the mint essential oil and the slough extract of Agkistrodon acutus produced in Yongzhou and their mixture were tested by the method of susceptibility disk test. Results showed that,20 components exist in the essential oil of Yongzhou mint,among which menthol (42.67%) and piperitone (27.30%) were the main components accounting for nearly 70% of the total content,and 7 other components such as α-pinene (3.65%), β-pinene (3.28%),limonene (5.63%),octanol (1.47%),isomenthol (7.88%),pulegone(1.35%) and menthyl acetate (2.70%) were more than 1%;meanwhile,there were two components of terpenes and dihydrocarcinol which were not detected in other producing areas’ mint essential oils were both more than 0.50%;The mint essential oil and the slough extract of Agkistrodon acutus produced in Yongzhou both showed antibacterial activity against Shigella dysenteriens and Staphylococcus aureus;mint essential oil perfomed bettrer against S. dysenteriens than S. aureus,while the slough extract showed no significant difference on antibacterial activity between two species of bacteria;the mixture of mint essential oil and slough extract perform better than both single prepration and the best mixing ratio was 1∶2 (mint∶slough,V∶V) with a same pre-mixing original concentration;the minimum inhibitory concentrations (MIC) against S. dysenteriens and S. aureus of the mixture were 15 and 10 mg/mL,respectively.The results could provide experimental basis for the collaborative development of two local characteristic animal and plant resources.