Taking Camellia sinensis‘Jin-guanyin’ as test material,glyphosate was quantitatively applyed on tea plants cultivation substrate.By observing the apparent phytotoxicity of tea leaves,and using ultra-high performance liquid phase tandem mass spectrometry to determine the pesticide residues (glyphosate and aminomethylphosphonic acid) and main quality components (free amino acids,catechins and alkaloids) in the tea leaves before (0 d) and after (7 d and 33 d) glyphosate applying,to explore the impact of glyphosate application on the quality and safety of tea production.The results showed that:glyphosate was applied for 33 days,and there was no obvious sign of phytotoxicity in tea leaves,but glyphosate and its metabolite aminomethylphosphonic acid were still detected;The total amount of free amino acids and catechins decreased after the application of glyphosate,while the total amount of alkaloids first increased and then decreased;Multivariate statistical analysis showes,there are obvious differences of theanine,caffeine,epigallocatechin gallate,glutamine,epicatechin gallate,epicatechin,arginine,aspartic acid,glutamic acid and epigallocatechin between the sample groups before and after glyphosate application.In summary,the application of glyphosate in tea gardens is not easy to cause phytotoxicity in the tea leaves,but it can remain in the leaves of tea plants for a long time and significantly change the content of free amino acids,catechins and alkaloids in tea leaves.