Based on the method of network pharmacology prediction and experimental verification,this article discusses the mechanism of reducing hypertension of Chinese herbal compound combined with hydrolyzed casein peptide.The main active ingredients of traditional Chinese medicine prescriptions and hydrolyzed casein peptides in TCM-ID database were screened.TCMID database,TCM@TAIWAN database,and select rutin,quercetin,epicatechin,procyanidin B2,α_s1-CN,α_s2-CN were used as the main active ingredient for network pharmacological analysis to screen the network targets of the main ingredients acting on cardiovascular diseases;combined with KEGG and Uniprot database,signal pathway and biological pathway analysis weer conducted to predict its mechanism of action.Based on network pharmacology prediction,an in vitro animal model was established to observe and study the antihypertensive effect and mechanism of Chinese herbal compound combined with hydrolyzed casein peptides on spontaneously hypertensive rats.The results of the network analysis showed that the prescriptions can exert anti-hypertensive effects by acting on the same target or different targets,and its mechanism of action was related to the regulation of the renin-angiotensin system,vascular endothelial tension factor-contraction factor system and other pathways,participating in the inflammatory response,endothelial cell protection and lipid biosynthesis and metabolism and other biological pathways.In other words,in animal models,by acting cGMP-PKG signaling athway,the prescription increased NO and AngII levels,and reduced ET-1,ALD,and TNF-α levels to reduce blood pressure,enhance immunity,and protect kidneys and so on,which verified the prediction results of network pharmacology,and comprehensively explained the antihypertensive mechanism of the prescription.