Mass spectrometry molecular networking is often used in the discovery and identification of novel natural products,drug metabolism and drug research and development.At present,molecular networks can connect compounds with similar secondary mass spectra into clusters,which have been widely used in the analysis of natural products.This review will briefly introduce the basic concepts of molecular networks and the steps of constructing molecular networks,as well as expounds the application of molecular network from five aspects:the identification and discovery of compounds;identification of metabolites of natural products;qualitative and quantitative analysis of chemical composition of natural products;screening,separation and preparation of active components of natural products;the biosynthetic pathway of natural products.