In this study,ebolavirus single glycoprotein of ebolavirus (EBOV-sGP) was used as the target,using diverse virtual screening technologies,such as drug-like evaluation,molecular docking,ADME prediction,binding free energy calculation,molecular dynamics simulation,etc.,in order to mine potential antiviral natural products from the database of traditional Chinese medicine.Taking toremifene as a positive control,four lead compounds,MOL006834,MOL000174,MOL002192,and MOL012524 with good drug-like properties were finally sorted.Their affinity values and interaction groups with EBOV-sGP are close to or similar to toremifene.The research results can promote the work of extracting,designing and experimentally synthesizing anti-ebolavirus compounds from natural products library of traditional Chinese medicine.