To study the metabolites of Cladosporium tenuissimum MT-35,an endophytic fungus isolated from Huperzia serrata.The compounds were separated and purified by silica gel column chromatography and semi-preparative HPLC.Ten compounds were isolated from the brown rice fermentation of the endophytic fungus C. tenuissimum MT-35,including 4-hydroxy-3-methoxy-2(1H)-quinolinone (1),acropyrone (2),lumichrome (3),(1H-indol-3-yl) oxoacetamide (4),1,6-dihydro-1-methyl-6-oxo-3-pyridinecarboxylic acid (5),uracil (6),ergosterol peroxide (7),ergosterol (8),9(Z),12(Z)-octadecadienamide (9),8-O-4-dehydrodiferulic acid (10).Except for compounds 7 and 8,other compounds were obtained from C. tenuissimum for the first time.Compounds 3,7,8 and 9 showed inhibitory activity against NO release from RAW 264.7 cells with IC₅₀ values of 36.8±1.0,13.9±0.2,36.6±0.0,53.2±1.4 μmol/L,respectively.Compound 3 showed inhibitory activity against acetylcholinesterase with an IC₅₀ value of 77.9±11.1 μmol/L.

"> Huperzia serrata, endophytic fungi, Cladosporium tenuissimum, structural identification, anti-inflammatory activities, inhibitory activity against acetylcholinesterase