To study the chemical constituents of Delphinium umbrosum Hand.-Mazz.,fourteen compounds were isolated from the ethanol extract of D. umbrosum by silica gel column chromatography. Their structures were identified by means of spectroscopic methods such as HR-ESI-MS,1D and 2D NMR,including thirteen lycoctonine C₁₉-type diterpenoid alkaloids:lycoctonine (1),anthranoyllycoctonine (2),majusine A (3),14-deacetylajadine (4),ajacine (5),14-deacetylnudicauline (6),methyllycaconitine (7),delsemines A and B (8),delavaine A free acid and delavaine B free acid (9),delavaines A and B (10),gyalanines A and B (11),umbrosumine C (12),umbrosumines A and B (13),and one isoquinoline alkaloid:S-glaucine (14). All compounds were isolated from this plant for the first time. The inhibitory effects of compounds (3-8,10-13) on lipopolysaccharide-induced NO production in mouse RAW 264.7 macrophages,and the anti-tumor effects of compounds 1-14 on mouse breast cancer 4T1 cells were tested,and the results showed that all the tested compounds had no obvious anti-inflammatory and anti-tumor activities.