To find the lipid-lowering and weight-loss components in Ilicis Cornutae Folium,a TLC-bioautographic method was used to disclose the active components with lipase inhibitory activity.The active components in TLC were quickly analyzed by in-situ TLC-EFISI-MSⁿ method to characterize their structures.As a result,10 spots on TLC-bioautogram with lipase inhibitory activity were rapidly analyzed,among which a total of 15 possible components were tentatively identified by TLC-EFISI-MSn.These components included nine ursane triterpenoids,one oleanolane triterpenoid,and five 18,19-seco triterpenoids. Among them,12 compounds contained in seven active spots were verified by co-TLC analysis with the reference substances.This method could quickly screen and characterize the active components with lipase inhibitory activity from Ilicis Cornutae Folium,based on which the screening and identification of active components in Ilicis Cornutae Folium can be conducted.