This study aimed to investigate the chemical constituents and anti-inflammatory activity in vitro from Gentiana davidii Franch..The chemical constituents of the ethyl acetate and n-butanol fractions of 70% ethanol extract in G. davidii were separated and purified by silica gel,ODS gel,and Sephadex LH-20 gel column chromatographies.Their structures were identified by physicochemical properties,spectroscopic analysis,as well as comparisons with the data reported in literature.Fifteen compounds were isolated and identified as ursolic acid (1),uvaol (2),ursaldehyde (3),28-norurs-12-en-3β-ol (4),oleanolic acid (5),β-amyrin-n-nonyl ether (6),vanillyl glycol (7),β-sitosterol (8),β-daucosterol (9),anemarrhenoside B (10),n-hexacosane (11),n-hentriacontyl alcohol (12),1,5-bis β-D-glucopyranosyloxy-2-(3′,3′-dimethylallyl)benzene (13),2,4-di-tert-butylphenol (14),2,2′-oxybis(1,4-di-tert-butylbenzene) (15).Compounds 7,14 and 15 were first isolated from Gentiana.All the compounds were first isolated from G. davidii. The compounds 1-15 were preliminarily examined for anti-inflammatory activity in vitro. Compared with the model group,compounds 1,5 and 9 significantly inhibited the production of NO in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.