Lonicerae Flos has the function of clear away heat and toxic material and treat the exopathogenic wind-heat,which has a broad application prospect.Only chlorogenic acid,macranthoidin B and dipsacoside B were used as quality evaluation indexes of Lonicerae Flos in Chinese Pharmacopoeia at present,which can′t comprehensively evaluate the quality of Lonicerae Flos.In this study,we have establelished the HPLC fingerprints of Lonicerae Flos,and evaluated the similarity of 29 batches of Lonicerae Flos.We screened the potential Q-marker of Lonicera Flos by orthogonal partial least squares discriminant analysis (OPLS-DA).We analyze and verify the effectiveness of the potential Q-Marker of Lonicera Flos by constructing a "component-target-pathway" network diagram through network pharmacology.The research results showed that chlorogenic acid,isochlorogenic acid A,neochlorogenic acid,sweroside,isochlorogenic acid B,macranthoidin B,caffeic acid,and dipsacoside B can be used as quality markers of Lonicera Flos,which can provide scientific basis for the quality control and evaluation of Lonicera Flos.