Achyranthes aspera L. is a widely used medicinal plant with many effects.In order to study the anti-cancer effect of its alcohol extract,the alcohol extraction process from A. aspera was optimized by Box-Behnken response surface method,and the cytotoxicity and cell scratch test of human lung cancer A549 cells were carried out in vitro.The optimum extraction conditions were as follows:extraction temperature 90 ℃,liquid-solid ratio 40∶1,extraction time 2 h.Under these conditions,the extraction rate of alcohol extract from A. aspera was 9.23%.The results of cytotoxicity test showed that the survival rate of A549 cells decreased rapidly with the increase of alcohol extract concentration.When the concentration of alcohol extract was 256 μg/mL,the inhibition rate of A549 cells was 94.65%.The cell scratch results showed that alcohol extract had a certain inhibitory effect on the migration of A549 cells,which can provide a reference for the study of the medicinal properties from A. aspera.