NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2023, Vol. 35 ›› Issue (9): 1469-1479.doi: 10.16333/j.1001-6880.2023.9.001

    Next Articles

Study on HPLC fingerprint and chemical pattern recognition of triterpenoids in Poriae Cutis and White Poria

ZHAN Hui-hui1,2,3,DING Chan1,2,3,PENG Si-yuan1,2,3,LIU Yuan1,2,3,MENG Jun-hua1,2,3,XIAO Zuo-wei1*,CUI Pei-wu1,2,3*   

  1. 1College of Pharmacy,Hunan University of Chinese Medicine;2Research Lab of TCM Property & Efficacy,Tier 3,National Administration of TCM;3Mycomedicine Research Lab,Hunan University of Chinese Medicine,Changsha 410208,China
  • Online:2023-09-22 Published:2023-09-21


In this study,HPLC analysis combined with chemical pattern recognition was employed to establish the HPLC fingerprint related to triterpenoids in Poriae Cutis and White Poria,which can provide a reference for quality control and standard establishment of decoction pieces derived from mycomedicine Poria cocos.HPLC method was adopted and performed on an Agilent 5 TC-C18 (2) column (250 mm × 4.6 mm,5 μm).The mobile phase was consisted of acetonitrile and 0.3% phosphoric acid solution,and gradient elution procedure was employed with the flow rate setting at 1.0 mL/min.The column temperature was set at 25 °C,the detection wavelength was set at 242 and 203 nm,and the injection volume was 10 μL,respectively.To establish the HPLC fingerprint and chemical pattern recognition of Poriae Cutis and White Poria,cluster analysis (CA),principal component analysis (PCA) and similarity evaluation were adopted to process the experimental data,the similarity and difference of Poriae Cutis and White Poria samples were analyzed subsequently.Finally,the key chromatographic peaks representing triterpenoids were pointed out from the established HPLC fingerprint.Among the common chemical components showed in the chromatograms,six peaks related to poricoic acid B (peak 2),dehydrotumulosic acid (peak 3),poricoic acid A (peak 4),dehydropachymic acid (peak 9),pachymic acid (peak 10),and dehydrotrametenolic acid (peak 10) were indentified,respectively.The similarity of tested decoction pieces from the same part of P. cocos sclerotium were all above 0.90.According to CA and PCA data,all the decoction pieces analyzed in this study can be divided into two groups representing different medicinal parts of P. cocos sclerotium.Seven common chromatographic peaks including peak 3 (dehydrotumulosic acid),peak 5,peak 6,peak 8,peak 9 (dehydropachymic acid),peak 10 (pachymic acid) and peak 11 were essential for the quality evaluation of Poriae Cutis and White Poria,the other eight chromatographic peaks including peak 1,peak 2 (poricoic acid B),peak 4 (poricoic acid A),peak 7,peak 12 (dehydrotrametenolic acid) and peaks 13 to 15 could be used as the characteristic identification peaks for evaluating the quality of Poriae Cutis according to the loading scatter analysis of PCA data.The characteristic difference deduced from the 15 common chromatographic peaks of White Poria samples was smaller than that of Poriae Cutis samples.The established HPLC fingerprint of Poriae Cutis and White Poria combined with chemical pattern recognition can provide a scientific reference for the quality control and evaluation of samples or products derived from P. cocos .

Key words: Poria cocos, triterpenoids, fingerprint, cluster analysis, PCA, quality control

CLC Number: