Abstract: The experiment concerning in vitro effect of Acanthopanax senticosus polysaccharide (ASPS) on signal transduction of peripheral lymphocyte was conducted to investigate the mechanism of immunoregulation effect of ASPS.Peripheral blood lymphocyte suspension was prepared,and T,B cells of which were separated and co-incubated with or without various concentrations of ASPS (40,80,160,320 μg/mL) separately to determine lymphocyte proliferation measured by MTT assay.Anti-TLR4 was designed to discuss the lymphocyte receptor.The results showed that ASPS significantly improved the T cell proliferation and the concentration of IL-2 secreted by lymphocyte (P<0.05),but had no significant effect on the B cell proliferation and the concentration of IL-4 (P>0.05).ASPS induced the production of TNF-α,NO,iNOS as well as NF-κB secreted by lymphocyte when TLR4 antibody was not supplemented in culture solution (P<0.05),and these indices were not affected when TLR4 antibody was added in the culture solution (P>0.05).These results suggested that T lymphocyte probably as one of the direct target cells was mediated by ASPS through TLR4/NF-κB signaling pathway.

Key words: Acanthopanax senticosus polysaccharide, lymphocyte proliferation, signal molecules, piglets