NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2014, Vol. 26 ›› Issue (2): 263-267.

• Article • Previous Articles     Next Articles

Effects of N-carbamyglutamate on Intestinal Microflora of Weaned Huanjiang Mini-pigs

ZHOU Xiao-li1,2, LIAN Guo-qi1, KONG Xiang-feng1,3*, WANG Sheng-ping1, YIN Yu-long1,2   

  1. 1 Key Laboratory of Agro-ecological Processes in Subtropical Region and Hunan Engineering and Research Center of Animal and Poultry Science,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China;2 State Key Laboratory of Food Science and Technology,College of Life Science and Food Engineering,Nanchang University,Nanchang 330047,China; 3 Huanjiang Observation and Research Station for Karst Ecosystems,Chinese Academy of Sciences,Huanjiang 547100,Guangxi
  • Online:2014-02-28 Published:2014-12-12

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Abstract: This study was conducted to investigate the effects of N-carbamoylglutamate(NCG) on intestinal microflora of weaned mini-pigs.Twelve Huanjiang mini-pigs(3.17±0.21 kg of average body weight;weaned at 21 days of age) were assigned randomly into two groups with six replicates in each group.The pigs were fed with a basal diet or 0.1% NCG-supplemented diet for 14 days.At the end of the trial,the contents of ileum and cecum were collected to determine the intestinal microflora by T-RFLP,PCR/DGGE and RT-qPCR technique.The results showed that diet supplemented with NCG increased(P<0.05) the intestinal microflora diversity in ileum,and population of Bifidobacterium and Lactobacillus in ileum and cecum,while decreased(P<0.05) Escherichia in ileum and cecum and Streptococcus in cecum when compared with the control group.These findings suggested that diet supplemented with NCG enriched beneficial intestinal microflora,but suppressed bacterial pathogens in Huanjiang mini-pigs,which effectively promoted the diversity of intestinal microflora.

Key words: N-carbamoylglutamate, Huanjiang mini-pigs, intestinal flora

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