Abstract: The objective of this study was to investigate the impact of lentinan on the growth of glioma in rats and inhibition of glioma cell in vitro. For the animal experiment, normal saline and different doses of lentinan were gavaged to the model rats for 30d.The changes of tumor tissue were then observed by immunohistochemical methods.For the in vitro experiment,the glioma cells were treated by lentinan for 24,48 and 72h.The activity,apoptosis and cycle of glioma cell were then detected by flow cytometry.The experimental results showed that the tumors volume dwindled in sample group compared with the control group;Coloration of nucleus was lighter in the sample group;Number of new blood vessels was reduced.For the in vitro experiment,the apoptosis rate of glioma cells of the 40 mg/L and 80 mg/L lentinan groups were significantly higher than that of control group (P<0.01) after 72 h of treatment;The number of G0/G1 phase cells of the 20,40,80 mg/L lentinan groups,were increased significantly (P<0.05) compared with that of the control group after 72 h of treatment.These results showed that the lentinan can inhibit the growth of glioma in rats,and cause the apoptosis of glioma cells in vitro.

Key words: lentinan, glioma, apoptosis, drug effects