Abstract: The purpose of this study was to determine the total flavonoids and dihydroquercetin contents in Picea asperata Mast.,and to further investigate its antioxidant activity.The sodium nitrite-aluminum nitrate colorimetric method and HPLC method were used to determine the contents of total flavonoids and dihydroquercetin, respectively.The DPPH scavenging,ABTS scavenging and reducing power assays were used to determine antioxidant ability of the total flavonoids of P.asperata. As the result,the total flavonoids content in P.asperata was 2.78 g/100 g,in which the dihydroquercetin content was 0.374%.In addition,the total flavonoids of P.asperata showed an obvious eliminating effect against DPPH and ABTS free radicals,at the concentration level of 0.5 mg/mL.The eliminating rate was 78.7% and 82.3%, respectively. Its reducing power was also strong.

Key words: Picea asperata Mast., total flavonoids, dihydroquercetin, content determination, antioxidant