Abstract: This paper aimed to establish a quantitative analysis model for total iridoid glycosides and phenylethanoid glycosides in leaves of Rehmannia glutinosa.The content of total iridoid glycosides and phenylethanoside in 128 samples of Rehmannia glutinosa leaves with different texture and different growth stages were determined by UV-Vis spectrophotometry,and it was used as the base value,combined with the near infrared spectra of leaves of Rehmannia glutinosa.The quantitative analysis models of total iridoid glycosides and phenylethanoside in leaves of Rehmannia glutinosa were established by using TQ 8.0 analysis software and partial least squares (PLS).The internal cross validation determination coefficient (R²) was 0.998 19,the correction mean variance (RMSEC) was 0.089 9,the root mean determination coefficient (R²) and the correction mean variance (RMSEC) of the quantitative calibration model for total iridoid glycosides in leaves of Rehmannia glutinosa were 0.972 1 and 0.125 9 respectively.The root mean square error predication was 0.095 4 and the root mean square error of cross validation(RMSECV) was 0.869 4.There was no significant difference between the predicted value and the actual value.The quantitative model can be used to determine the content of total iridoid glycosides and total phenylethanol glycosides in leaves of Rehmannia glutinosa.

Key words: Rehmannia glutinosa, near-infrared spectroscopy, total iridoid glycosides, total phenylethanoid glycosides, rapid determination