NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2025, Vol. 37 ›› Issue (4): 678-686. doi: 10.16333/j.1001-6880.2025.4.010 cstr: 32307.14.1001-6880.2025.4.010

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Quality evaluation of Fici Hirtae Radix from different regions in Guangxi based on HPLC fingerprint and UPLC-MS/MS multi-index component content determination

HUANG Chun-yan1, HE Min1, HE Qiu-yun1, LIU Xian-fu2*, MO Yuan-yao1, ZHOU Yi1, MO Yong-fu1, CHEN Ru-xu2*   

  1. 1Qinzhou Inspection and Testing Centre, Qinzhou 535000, China; 2Faculty of Chinese Medicine Science Guangxi University of Chinese Medicine, Nanning 530222, China
  • Online:2025-04-30 Published:2025-04-27

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Abstract:

To establish a quality control method for multi-index components of Fici Hirtae Radix. Established it’s fingerprint by HPLC, and the HPLC fingerprinting was established by “Similarity Evaluation System for Chromatographic Fingerprinting in Chinese Medicine (2012A version)”, and the samples were analysed by SPSS 22.0 for the clustering analysis (CA) and the principal component analysis (PCA), and the content of multi-index components was determined by UPLC-MS/MS. The HPLC fingerprint identified 24 common peaks, of which seven perks were identified as psoralen, quercetin, naringenin, luteolin, bergapten, apigenin, and aconitine. The similarities were 0.990-0.999, and the samples were divided into three categories. The experimental results showed that Fici Hirtae Radix contained eight components by UPLC-MS/MS, including psoralen, quercetin, naringenin, luteolin, bergapten, kaempferol, apigenin, and aconitine. The eight components exhibited excellent linear relationships within their respective mass concentration ranges, the average recoveries were 96.88%-101.0%, and the RSD were 1.2%-1.8%.The CA results showed that the 12 batches of samples were clustered into three groups, the PCA results showed that the eigenvalue of the first principal component (psoralen) was 11.89, and the variance contribution rate was 99.08%, which indicated that this principal component could be regarded as a quality marker for the Fici Hirtae Radix. The content of eight components in 12 batches of samples were 0.085 8-1.11, 2.51×10-6-5.34×10-4, 1.24×10-3-0.117, 2.30×10-4-3.98×10-2, 0.011-0.507, 3.36×10-6-8.17×10-4, 2.52×10-3-7.92×10-2, 5.13×10-5-5.85×10-4 mg/g. There were certain differences in the content of each component in the 12 batches of samples. In conclusion, the established method have good accuracy, stability, and reliability, and it can provide a scientific reference for the quality control of Fici Hirtae Radix.

Key words:

Fici Hirtae Radix, HPLC, UPLC-MS/MS, fingerprint, multi-index component content determination

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