This study aims to improve the processing efficiency and quality of Asari Radix et Rhizoma,optimize the fresh-cut technology of Asari Radix et Rhizoma.The single factor test was used to evaluate the amounts of volatile oil,asarinin,and alcohol-soluble extract under various drying methods,cutting length,drying temperature,and drying time using six-year-old Asari Radix et Rhizoma as raw material.An orthogonal experiment and the entropy weight approach were used to determine the most suitable process for fresh-cut processing of Asari Radix et Rhizoma and to assess the analgesic and anti-inflammatory effects of fresh-cut Asari Radix et Rhizoma pieces.The study concluded that the most suitable conditions for fresh-cut processing of Asari Radix et Rhizoma were blast drying,cutting length of 2.0 cm,drying temperature of 40 ℃,drying time of 3 h.Fresh-cut aqueous extracts of Asari Radix et Rhizoma can significantly increase the pain threshold of mice and inhibited the number of twitches in mice with 43.64% inhibition rate.Fresh-cut aqueous extracts of Asari Radix et Rhizoma at 25 μg/mL,50 μg/mL,and 100 μg/mL inhibited NO secretion,inducible nitric oxide sythase and prostaglandin-endoperoxide synthase 2 protein expression in lipopolysaccharide-induced mice monocyte macrophage RAW 264.7 cells.The experimental results showed that the fresh-cut Asari Radix et Rhizoma pieces have obvious analgesic and anti-inflammatory effects,which can better preserve the efficacy and quality of Asari Radix et Rhizoma and improve the processing efficiency.It provides reference for the research and application of the integration of Asari Radix et Rhizoma processing and decoction piece processing.It is feasible to use the fresh-cut processing in the production of Asari Radix et Rhizoma,which can provide a reference for the research and application of the integration of Asari Radix et Rhizoma processing pieces and tablet concocting.