Abstract: The whole plant of Iris japonica Thunb(Iridaceae) were investigated on their chemical constituents and bioactivities,eighteen compounds were isolated from an ethanolic extract from the whole plants of I. japonica by a combination of various chromatographic techniques including column chromatography over silica gel and Sephadex LH-20 and reversed-phase HPLC.Their structures were determined by spectroscopic analysis including MS and NMR data.These compounds were identified as(+)-(7S,8R,7′E)-4-hydroxy-3,5′-dimethoxy-4′,7-epoxy-8,3′-neolign-7′-ene-9,9′-diol9′-ethyl ether(1),syringaresinol(2), erythroguaiacylglycerol-β-O-4′-coniferylether(3), threo-guaiacylglycerol-β-O-4′-coniferylether(4),4-O-β-d-(6-O-vanilloylglucopyranosyl) vanillic acid(5),4-［6-O-(4-hydroxy-3,5-dimethoxybenzoyl)-β-d-glucopyranosyloxy］-3-methoxybenzoic acid(6),scroneoside B(7),belalloside B(8),scroneoside A(9),apocynin(10),7-O-methylnaringenin(sakuranetin)(11),rhamnoeitrin(12),iristectorigenin B(13),genistein(14),5,7,4′-trihydroxy-6,8-dimethoxyisoflavone(15),irigeni(16),8hydroxytectrigenin(17),3′-O-methylorobol(18).Compounds 1-18 were obtained from this plant for the first time.Compounds 1,2,5,6,15 were obtained from this genus for the first time.The isolated compounds were screened for hepatoprotective,anti-inflammatory,antioxidant activities.

Key words: Iris japonica, chemical constituent, hepatoprotective, anti-inflammatory, antioxidant