The ¹³C NMR and CD spectral characteristics of tetrahydrofuran lignans from Forsythia suspensa had been studied.Seven lignans were isolated from the 50% EtOH extract of F.suspensa by a combination of various chromatographic techniques including column chromatography over macroporous resin,silica gel,Sephadex LH-20 and reversed-phase HPLC.Their structures were identified as (+)-8-hydroxyepipinoresinol-4-O-β-D-glucopyranoside (1),(+)-8-hydroxyepipinore-sinol-4′-O-β-D-glucopyranoside (2),(+)-pinoresinol-4-O-β-D-glucopyranoside (3),(+)-epipinoresi-nol-4-O-β-D-glucopyranoside (4),(+)-epipinoresinol-4′-O-β-D-glucopyranoside (5),(+)-phyllrin (6) and (+)-pinoresinol (7) on the basis of chemical and spectral analysis.The ¹³C NMR and CD characteristics of the configuration of tetrahydrofuran lignans were summarized in this paper.