NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2020, Vol. 32 ›› Issue (9): 1576-1581.doi: 10.16333/j.1001-6880.2020.9.016

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Synthesis of silybin complexes with trace elements and their anti-bacterial and anti-tumor activities

LIU Yan-ji1,TU Li-hua1,YU Jia2*,LI Jin1,YU Wei-qiang1,ZHANG Li-ping1,WANG Xiao-ying1   

  1. 1Nanchang Health School,Nanchang 330006,China;2Third Affiliated Hospital of Nanchang University,Nanchang 330008,China

  • Online:2020-09-28 Published:2020-09-25


Six new Chinese medicine complexes of silybin calcium,iron,zinc,selenium,cobalt and chromium were synthesized and characterized by IR,UV,LC-MS,EDTA complexometric titration and elemental analysis.The results showed that coordination ratios of these six silybin complexes were 2∶1 (silybin ratio to trace elements).The safety of complexes was evaluated by acute toxicity test on mice,and found that no obvious toxicity of these complexes in vivo.Furthermore,the anti-bacterial and anti-tumor activities were determined respectively by double dilution method and MTT assay.For the anti-bacterial assay,nine types of bacteria were used in this experiment and the minimum inhibitory concentration (MIC) were determined.The results showed that the anti-bacterial effect of silybin was increased to 2 to 4 times after reaction with the six trace elements.For the anti-tumor assay,experiments were carried out in 5 types of cancer cells and the half inhibitory concentration (IC50) of the six complexes was determined.The results showed that the anti-tumor effect of these complexes was increased to 1.11-1.53 times compared with that of Silybin.In conclusion,the anti-bacterial and anti-tumor activities of silybin are elevated after the formation of complexes with the trace elements.

Key words: silybin, trace elements, complex, antibiosis, antitumor

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