Dried powder of whole herbal of Potentilla discolor Bge.was isolated and purified in this work.Accelerate solvent extraction,polyamide column chromatography,vacuum silica gel column chromatography and atmospheric pressure silica gel column chromatography and preparative high performance liquid chromatography coupled with mass spectrometry technology were utilized.The chemical structure of the separated compounds were identified by MS,1H NMR,13C NMR,2D NMR and some other spectral analysis methods.Six compounds were isolated and identified as kaempferol-3-O-(6′′-O-E-p-coumaroyl)-β-D-glucopyranoside (1),apigenin (2),maslinic acid (3),3′-O-methylellagic acid-4-O-β-D-glucopyranoside (4),β-daucosterol (5),apigenin-7-O-β-D-glucuronoside (6).Among them,compound 4 was isolated from P. discolor for the first time.