In order to quickly analyze and identify the chemical components of the alcohol extract of Rosa sertata×Rosa rugosa,ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used in this study,Waters ACQUITY UPLC HSS T3 column (100 mm×2.1 mm,1.8 μm) was used with The mobile phase is 0.1% formic acid aqueous solution (A) and 0.01% formic acid acetonitrile solution (B) and gradient elution.The MSE data were collected under the negative ion condition of electrospray ion source (ESI).Sixty-three main components were detected according to the exact relative molecular mass,mass spectrometry fragmentation information,and related literature and reference materials,including 26 flavonoids,11 polyphenols(4 phenolic acids and 7 tannins),7 triterpenoids,4 phenylpropanoids,8 organic acids,1 iridoid terpene,1 volatile oil and 5 unidentified components.Twenty-eight compounds such as pectolinarin,melilotoside and asiatic acid were identified in this genus for the first time.UPLC-Q-TOF-MS method was used to further improve the chemical constituents of Rosa sertata×Rosa rugosa,which provided a reference basis for the basic study of pharmacodynamic substances.