Abstract: The contents of arbutin in pear blossoms of different periods and different varieties were detected using the HPLC method with Hypersil BDS C₁₈ column (4.6 mm × 250 mm,5 μm),the mobile phase methanol :water (6:94,with 0.05% formic acid),and the detection wavelength 280 nm.The results showed that the calibration curve was linear in the range of 0.01~5.00 μg (r = 0.9999) and the average recovery was 97.7%.The content of arbutin in Yali pear blossom during the florescence was above 10 mg/g (fresh weight) in most cases,especially in blossom bud germination period,reaching a maximum of 35.7 mg/g.In the nine pear blossom samples from different varieties,the contents of arbutin were range between 3.5 mg/g to 10.5 mg/g.

Key words: pear flower, arbutin, HPLC