NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2024, Vol. 36 ›› Issue (2): 206-217.doi: 10.16333/j.1001-6880.2024.2.003

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Study on the mechanism of Tibetan medicine Myricaria germanica on ulcerative colitis

LI Xin-rui1,CHEN Hai-juan1,2*,GAO Yu-han1,XU Li1   

  1. 1Key Laboratory of Medicinal Animal and Plant Resources in Qinghai Tibet Plateau,School of Life Sciences,Qinghai Normal University;2Academy of Plateau Science and Sustainability,Xining 810008,China
  • Online:2024-02-23 Published:2024-02-23

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Abstract:

Based on network pharmacology,molecular dock and experimental verification,the effect and mechanism of Myricaria germanica in the treatment of ulcerative colitis (UC) were investigated.The UHPLC-Q-Exactive-Orbitrap-MS technique was used for the rapid analysis of the original phytochemical components of M.germanica;the core components,target genes and main pathways of M. germanica for the treatment of UC were predicted and screened based on the network pharmacology,and molecular docking validation was carried out on the core components and targets;and the therapeutic effects of M.germanica on UC were observed by the mouse model of UC induced by dextran sulfate sodium (DSS).Twenty-three active ingredients of M. germanica for the treatment of UC were screened and acted on 111 common targets;PPI network analysis showed that AKT1,TNF,CASP3,etc.might be the core targets;GO biological process mainly involved inflammatory response,immune response,etc.;KEGG signaling pathway mainly involved MAPK signaling pathway,TNF signaling pathway,etc.;molecular docking showed that TNF,CASP3 and other core targets could bind well with the core active components;M. germanica can improve colon length and colonic mucosal damage in DSS-induced UC mice,and reduce the levels of TNF-α and IL-6 in colon and serum. M. germanica exerts its therapeutic effects on UC by modulating TNF-α,PTGS2 and other targets through compounds such as rheic acid,isorhamnetin,ellagic acid,wedelolactone and desmethylwedelolactone.

Key words: Myricaria germanica, ulcerative colitis, UHPLC-Q-Exactive-Orbitrap-MS, network pharmacology, molecular docking

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