NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2014, Vol. 26 ›› Issue (2): 225-229.

• Article • Previous Articles     Next Articles

Analysis of Flavonoid Components of Vitis lanceolatifoliosa C.L

HU Song-mei1, LI Si-zhang2*, YU Hua-fang1   

  1. 1 Hunan Loudi Vocational and Technical College,Loudi 417000,China; 2 Hunan Jiuding Science and Technology Limited Company,Yueyang 414000,China
  • Online:2014-02-28 Published:2014-12-12

Abstract: In this study the flavonoid components in stems and leaves of Vitis lanceolatifoliosa C.L from Hengshan mountain and Shaoyang of Hunan province were determined and analyzed by HPLC and UV double beam spectrophotometer.The results indicated the main flavonoids in V.lanceolatifoliosa were dihydroflavones. The optimal TLC elution system used for the flavonoids identification was petroleum ether:ethyl acetate:formic acid at 3:12:1,and the kinds of flavonoids in Shaoyang samples were richer than that of Hengshan mountain samples.Flavonoids in tested samples were mainly dihydroflavone,flavonol(rutin),flavone,flavonoid glycosides.The content of rutin in samples from Shaoyang was 4.27 mg/g.

Key words: Vitis lanceolatifoliosa C.L, flavonoids, HPLC, UV double beam spectrophotometer

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