Abstract: To study the chemical constituents from the roots of Scrophularia ningpoensis Hems,the constituents were isolated by silica gel column chromatography,chromatorex chromatography,Sephadex LH20 chromatography and preparative HPLC.On the basis of spectral data and physiochemical properties,the structures of 15 known compounds were identified as:harpagoside (1),harpagide (2),ajugol (3),6-O-methycatalpol (4),geniposide (5),6-O-α-L-rhamnopyranosylaucubin (6),ningpogenin (7),liquiritigenin (8),di-2-ethyl hexyl phthalate (9),maltose (10),ursolic acid (11),glycerol (12),5-hydroxy methyl furfural(13),daucosterol (14) and β-sitosterol (15).Compound 3,6,8,9,10 and 12 were obtained from this plant for the first time.

Key words: Scrophularia ningpoensis, iridoid glycosides, Scrophulariaceae