Abstract: To observe the protective mechanism of “Tong Ping heart protection” formula for H9c2 myocardial cell injured by high glucose.Cellular damage model was developed using high glucose;cell survival rate was detected by MTT method;the cell mitochondria membrane potential was detected by LSCM to identify whether “Tong Ping heart protection” formula protected myocardial mitochondria by inhibiting the mitochondrial permeability hole(mitochondrial permeability transition pore,mPTP)open;p-JNK and p-GSK-3β were tested by western blot.The results showed that “Tong Ping heart protection” formula and JNK inhibitors both can block H9c2 myocardial cell injury damaged by high glucose.The cell survival rate raised,TMRE fluorescence wanes reduced,p-JNK expression decreased and p-GSK-3β expression increased in the “Tong Ping heart protection” formula(0.1 μg/mL)pretreatment(10 min)group.These results indicated that “Tong Ping heart protection” formula can protect mitochondrial against H9c2 cell damage induced by high sugar.The protection mechanism was probably through promoting its downstream factor GSK-3β phosphorylation through JNK pathway,thus inhibiting mPTP open.

Key words: diabetic cardiomyopathy;“Tong Ping heart protection&rdquo, formula;JNK pathway;myocardial injury