Abstract: Eight compounds were separated and purified from the ethyl acetate extracts of the fermentation broth of Antrodiella zonata by silica gel,RP-18,Sephadex LH-20 column chromatography,respectively.Their structures were identified as antrodiellone (1),22-E-7α-methoxy-5α, 6α-epoxyergosta-8 (14),22-dien-3β-ol (2), 3β,5α-dihydroxy-6β-methoxyergosta-7,22-diene (3), 6-bromo-1-H-indole-3-carboxylic acid ethyl ester (4), 6-bromo-1-H-indole-3-carboxylic acid (5), 4-hydroxyphenethyl alcohol (6), 5-(2-hydroxyethyl) furan-2-carboxaldehyde (7), and cyclo-(L-Leu–L-Val) (8).1 was a new compound.The other compounds were isolated from A. zonata for the first time.

Key words: Antrodiella zonata, chemical constituents, isolation, structural identification