To explain the mechanism of GRHP against rheumatoid arthritis (RA).RA rats’ model was constructed by collagen-induced arthritis (CIA) method and wind-cold-dampness stimulant condition,and screened by arthralgia index (AI) more than four.RA rats were classified to four groups such as model and three dose-ratio groups of GRHP except for normal group.Blood serum cytokine level of each rat was determined by ELISA approach,for example,rheumatoid factor (RF),C-reactive protein (CRP),thromboxane B2 (TXB2),prostaglandin E2 (PGE2) and prostacycline (PGI2).Whole blood COX-1 and COX-2 mRNA expression of each rats was determined by Real time quantitative PCR approach.As was shown in results,AI and blood serum cytokine level such as RF,CRP,PGE2 and PGI2 were markedly decreased,whole blood COX-2 mRNA expression reduced,body weight increased in the rats of dose-ratio groups by comparing with model group twenty-one day after intragastric administration.Therefore,GRHP has anti-inflammatory and anti-rheumatoid arthritis effects by downregulating COX-2 mRNA expression and decreasing proinflammatory factor level such as PGE2,PGI2,et al.

To investigate the mechanism of Resina Liquidambaris in the treatment of RA based on network pharmacology and molecular docking.According to TCMID platform and related literature,13 kinds of effective active ingredients of Resina Liquidambaris were required,and 27 target of active ingredients for RA treatment was searched from SwissTargetPrediction,TTD,NHGRI,GWAS database."Component-target network" and" target interaction network" was constructed based on Gephi software,which showed that Resina Liquidambaris play a role in the treatment of RA by acting on MAPK14,PTGS2 and other targets.Metascape was used for enrichment analysis of gene pathway.The results showed that Resina Liquidambaris play a role in the treatment of RA by acting on interleukin-2 related signal pathway and MAPK signal pathway,etc.After molecular docking of components and targets with LeDock,the higher docking scores are docking of M19 and CTSK,G6PD,MAPK14,PTGS2,M8 and G6PD,MAPK14,M6 and PTGS2,M23 and PTGS2.Therapeutic effect of Resina Liquidambaris on RA showed the characteristics of traditional Mongolian medicine as multi-components，multi-targets，and multi-pathways.

In this paper,the chemical constituents of Maca ethyl acetate extraction were studied,and its target proteins and possible pharmacological effects were predicted.In the experiment,silica gel column,Sephadex LH-20 column chromatography,HPLC semi-preparation were used to separate and purify the ethyl portion of Maca,and the structure was identified based on physical and chemical properties and spectral data.Then,PharmMapper database,AutoDock Tools,Pymol software and RCSB protein crystal database were used to perform target prediction and docking research on the isolated macaamide compounds.Four compounds were isolated and identified as 2-(3-hydroxyphenyl) acetonitrile (1),hexadecanamide (2),N-benzylpalmitamide (3),and 3-methoxy-4-hydroxybenzaldehyde (4).Compounds 1 and 4 were obtained from this plant for the first time.Results of molecular docking show that hexadecanamide binds well to lactyl glutathione lyase,S-methyl-5-thioadenosine phosphorylase,kinesin-like protein KIF11,and 3-oxoacyl-synthase 1,and compound 3 binds tightly to SEC14-like protein 2 and kinesin-like protein KIF11.Hence,compounds 2 and 3 are predicted to have potential pharmacological effects in treating rheumatoid arthritis,cataracts,and alleviating muscle spasms,and are worthy of further study.

To screen the active ingredients and potential mechanism of Strychnos nux-vomica L. in the treatment of rheumatoid arthritis by Chinese medicine network pharmacology.The TCMSP database was used to select the active ingredients and targets of S. nux-vomica,and then the Gene name annotation was performed using the UniProt database,and the disease-related targets were retrieved in the GeneCards database.The Rversion 3.6.2 software and Cytoscape 3.7.1 software was used to predict the targets and active components of S. nux-vomica. for RA treatment,and the interactions between proteins were evaluated using the STRING database.Finally,the BiocManager package in R language was used for GO analysis and KEGG pathway enrichment analysis.A total of 13 effective components of S. nux-vomica and 23 “Traditional Chinese medical” -“disease” targets were screened through the above process,and the core targets involved PTGS2,NR3C1,ESR1,MAOA,OPRM1,SLC6A4,etc.GO function enrichment analysis got 123 GO entries,and KEGG pathway enriched to obtain 34 signal pathways closely related to the onset of rheumatoid arthritis,such as interleukin (IL) - 17 signaling pathways,tumor necrosis factor (TNF) signaling pathway,the NF-κ B signaling pathways,toll-like receptors signaling pathways,apoptosis pathway,hypoxia inducing factor (HIF)-1 signaling pathways,T cell signaling pathways,B cell signaling pathways,MAPK signaling pathways,VEGF signaling pathways,JAK-STAT signal pathway and osteoclast differentiation pathway.This study predicts the material basis and potential mechanism of S.nux-vomica in the treatment of rheumatoid arthritis,and provides a theoretical basis for the further development of S.nux-vomica.