关键词: 两面针, 别隐品碱, 超声, 酶法, 工艺

Abstract: In this study,the ultrasonic-assisted extraction technology for allocryptopine from Zanthoxylum nitidum was investigated.Extraction with acidic solvent and pretreatment performance with enzymes were compared.The effects of ultrasonic power,times of extraction and solvent volume on extraction performance were optimized by orthogonal test.Extraction duration was optimized in dynamic process,with extraction yield of allocryptopine as the evaluation index.The results showed that the extraction yield of allocryptopine with neutral solvent was better than that with acidic (pH≤3) solvent.The optimal extraction process was as follows: Z.nitidum powder was pretreated with cellulase (1:250) and pectinase (1:250) for 30 min in 3 times volume of acetic acid-sodium acetate buffer (pH 5) at ambient temperature (≥30 ℃),and then extracted 3 times by ultrasonic wave (250 W) with 60% ethanol as solvent,extracted 18 min with 7 times solvent at the first time,extracted 12 min with 3 times solvent at the second time,extracted 12 min with 2 times solvent at the third time.Under the optimized extraction procedure,the extraction yield of allocryptopine was 89.5%.The optimized extraction process is economic,efficient,energy and time saving.It provides experimental basis for industrial production.

Key words: Zanthoxylum nitidum, allocryptopine, ultrasonic, enzymatic method, technology