天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (7): 1055-1059.doi: 10.16333/j.1001-6880.2016.7.012

• 研究简报 • 上一篇    下一篇

白肉榕叶提取物的生物活性及化学成分研究

韦乾蔚1，陈学理1，赵一燕1，古玉华1，徐汉虹2，李晶晶1,2*   

  1. 1 桂林医学院公共卫生学院，桂林 541004；2华南农业大学 亚热带农业生物资源保护与利用国家重点实验室 天然农药与化学生物学教育部重点实验室，广州 510642
  • 出版日期:2016-07-30 发布日期:2017-01-17

Bioactivities and Chemical Constituents of Ficus vasculosa Leaves Extracts

WEI Qian-wei1,CHEN Xue-li1,ZHAO Yi-yan1,GU Yu-hua1,XU Han-hong2,LI Jing-jing1,2*   

  1. 1 School of Public Health,Guilin Medical University,Guilin 541004,China;2 State Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources/Key Laboratory of Natural Pesticide and Chemical  Biology,Ministry of Education,South China Agricultural University,Guangzhou 510642,China
  • Online:2016-07-30 Published:2017-01-17

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摘要： 本文以白肉榕叶为研究对象，采用药剂浸泡法和平板涂布法分别测定了白肉榕乙醇粗提物对蚕豆蚜、致倦库蚊和福寿螺的毒杀活性以及对蜡样芽胞杆菌、大肠杆菌、伤寒杆菌、痢疾志贺氏菌和金黄色葡萄球菌5种供试菌的抑菌活性。结果表明，白肉榕乙醇粗提物对蚕豆蚜、致倦库蚊和福寿螺有生物活性，而对上述5种供试菌没有抑菌活性。采用液-液萃取法对白肉榕乙醇粗提物进行萃取分离，并对得到的石油醚萃取物、乙酸乙酯萃取物和水相3种不同极性部位进行生物活性测定。生物测定结果表明，白肉榕叶的活性成分主要存在于石油醚萃取物和乙酸乙酯萃取物中。从乙酸乙酯萃取物中进一步分离得到1个活性成分，经核磁共振氢谱（1H NMR）和碳谱（13C NMR）鉴定为桦木醇（betulin）。该化合物对致倦库蚊3龄幼虫具有较好的杀虫活性，24 h的LC50值为16.44 mg/L。

关键词: 白肉榕, 叶, 生物活性, 化学成分

Abstract: The bioactivity and antibacterial activity of ethanol extract from Ficus vasculosa leaves were tested by reagent impregnation method and spread-plate method, respectively. The results showed that,ethanol extract of F.vasculosa possessed bioactivity against Aphis fabae, Culex quinquefasciatus and Pomacea canaliculata, but exhibited no antibacterial activity against Bacillus cereus, Escherichia coli, Salmonella typhi, Shigella dysenteriae and Staphyloccocus aureus. The ethanol extract of F.vasculosa leaves were preliminarily partitioned using liquid-liquid isolation method,to give petroleum ether fraction,EtOAc fraction and H2O fraction,respectively.The further bioassays results suggested that the active constituents of F.vasculosa leaves existed in petroleum ether fraction and EtOAc fraction.One active compound was isolated from the EtOAc fraction through further phytochemical investigation,its structure was identified as betulin by 1H NMR and 13C NMR.Betulin exhibited potential insecticidal activity against the 3rd instar larvae of C.quinquefasciatus, with LC50 value of 16.44 mg/L after treatment in 24 h.

Key words: Ficus vasculosa, leaf, bioactivity, chemical constituent

中图分类号: 

R284.2 S482.1

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