关键词: 木犀草素, 肺癌, H460细胞凋亡, miR-34a-5p, p53

Abstract: To investigate the molecular mechanism for the induction of apoptosis by luteolin in human lung cancer H460 cells,and to provide a new scientific basis for its treatment of lung cancer.H460 cells in logarithm growth phase were selected and randomly divided into various groups:a blank control group and different concentrations of luteolin (LTL) groups.The cell viability was detected by CCK-8 assay,while the cell apoptosis was measured by flow cytometry analysis.The mRNA expression of lung cancer related genes in H460 cells were determined by PCR array.The protein levels of p53,p21,Bax and Bcl-2 were detected by Western blot.The expression levels of microRNA-34a (miR-34a) in H460 cells were assessed by qRT-PCR.Then,the miR-34a-5p was transfected into H460 cells,to observe its effects on cell apoptosis and related protein expression.The results showed that luteolin exhibited the anti-cancer effect in H460 cells through the induction of cell apoptosis and inhibition of cell proliferation,which may be mediated through the activation of the p53 signal pathway,up-regulation of miR-34a-5p,and subsequent regulation of apoptosis-related protein expression such as Bax/Bcl-2.

Key words: luteolin, lung cancer, H460 cell apoptosis, miR-34a-5p, p53