天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (增刊1): 130-135.doi: 10.16333/j.1001-6880.2020.S.021

• 开发研究 • 上一篇    下一篇

响应面法优化柠条锦鸡儿花中总黄酮超声提取工艺

马玉梅1,2，陈涛1，杨雪1,2，申诚1,2，闫淑萍1,2，李玉林1*   

  1. 1中国科学院西北高原生物研究所，西宁 810008；2中国科学院大学，北京 100049
  • 出版日期:2020-06-28 发布日期:2020-07-21
  • 基金资助:
    青海省重点研发与转化计划（2019-SF-123）

Optimization of extraction of total flavonoids from Caragana korshinskii Kom.with ultrasound technology by response surface analysis

MA Yu-mei1,2,CHEN Tao1,YANG Xue1,2,SHEN Cheng1,2,YAN Shu-ping1,2,LI Yu-lin1*   

  1. 1Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China;2 University of Chinese Academy of Sciences,Beijing 100049,China

  • Online:2020-06-28 Published:2020-07-21

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摘要：

本文以青海地区柠条锦鸡儿花为研究对象，用超声波辅助提取柠条锦鸡儿花中总黄酮，在选取提取温度、乙醇浓度、超声时间、料液比做单因素试验的基础上，采用Box-Behnken响应面法优化柠条锦鸡儿花中总黄酮提取工艺。结果表明：柠条锦鸡儿花总黄酮最优提取工艺为：提取温度80 ℃，乙醇浓度50%，超声时间60 min，料液比1∶20。应用优化后的提取工艺进行3组验证实验，总黄酮的得率为31.38 mg/g，RSD为0.17%。Box-Benhnken响应面法优化柠条锦鸡儿花中总黄酮的提取工艺稳定、可行。

关键词: 柠条锦鸡儿花, 总黄酮, 响应面分析法, 超声提取

Abstract:

In this paper,Caragana korshinskii Kom.flowers in Qinghai region was taken as the research object.The total flavonoids in C. korshinskii.flowers were extracted with the aid of ultrasound.On the basis of single factor experiments on extraction temperature,ethanol concentration,ultrasonic time and solid-liquid ratio,Box-behnken response surface method was used to optimize the extraction process of total flavonoids from C. korshinskii flowers.The results showed that the optimal extraction process for total flavonoids from C. korshinskii. flowers were as follows:extraction temperature 80 ℃,ethanol concentration 50%,ultrasonic time 60 min,and material-liquid ratio 1∶20.The optimized extraction process was used to perform three sets of verification experiments.The total flavonoid yield was 31.38 mg/g and the RSD was 0.17%.Box-Benhnken response surface method for the optimization of extraction of total flavonoids from C. korshinskii is stable and feasible.

Key words: Caragana korshinskii Kom. flowers., total flavonoids;response surface analysis;ultrasonic extraction

中图分类号:  R284.2

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