天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (4): 623-629.doi: 10.16333/j.1001-6880.2022.4.010

• 研究简报 • 上一篇    下一篇

牡荆素对1型糖尿病小鼠体内抗氧化酶活性及相关基因表达的影响

张泽鹏，徐兴军*，刘佳人，张伟伟，邵淑丽   

  1. 齐齐哈尔大学生命科学与农林学院 抗性基因工程与寒地生物多样性保护黑龙江省重点实验室，齐齐哈尔 161006
  • 出版日期:2022-04-29 发布日期:2022-04-29
  • 基金资助:
    黑龙江省省属本科高校基本科研业务费面上项目（135 509133）；黑龙江省省属高等学校基本科研业务费科研项目（YSTSXK2018）；齐齐哈尔大学研究生创新科研项目（YJSCX2019046）

Effects of vitexin on antioxidant enzyme activity and related gene expression in type 1 diabetic mice

ZHANG Ze-peng,XU Xing-jun*,LIU Jia-ren,ZHANG Wei-wei,SHAO Shu-li   

  1. Genetic Engineering and Winter Biodiversity Conservation Key Laboratory of Heilongjiang Province,School of Life Sciences and Agriculture and Forestry,Qiqihar University,Qiqihar 161006,China
  • Online:2022-04-29 Published:2022-04-29

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摘要： 为探究牡荆素对1型糖尿病小鼠的抗氧化作用，将84只4周龄SPF级雄性ICR小鼠按体质量随机分为空白对照组、水模型对照组、DMSO模型对照组、牡荆素高、中、低剂量组、阳性对照组，组间体质量差异不显著。模型构建成功并连续灌胃不同剂量牡荆素4周后，试剂盒法测定小鼠血清和肝脏组织中SOD、GSH-PX、T-AOC酶活性及MDA含量；荧光定量PCR测定抗氧化基因SOD-1、SOD-2、GPX-1、GPX-4表达量。结果表明，牡荆素可极显著提高Ⅰ型糖尿病小鼠SOD、T-AOC、GSH-PX酶活性，极显著降低MDA含量，显著上调SOD-1、SOD-2、GPX-1、GPX-4基因的表达量。牡荆素可提高1型糖尿病小鼠的抗氧化能力。

关键词: 牡荆素, 1型糖尿病, 抗氧化, 基因表达

Abstract:

To investigate the antioxidant effect of vitexin on type 1 diabetic mice,84 four-week-old SPF-grade male ICR mice were randomly divided into different control groups based on body weight of mice in this study,including blank control group,water model control group,DMSO model control group,model groups with high-,medium- and low-dose of vitexin,and positive control group.The difference in body weight of mice among control groups was not significant.After successful modelling and continuous gavage for mice with different doses of vitexin for 4 weeks,enzyme activities (SOD,GSH-PX and T-AOC) and MDA content in serum and liver tissues of mice were measured by the reagent kit method;the expression of antioxidant genes SOD-1,SOD-2,GPX-1 andGPX-4 were measured by quantitative fluorescence PCR.The results have shown that vitexin can highly significantly increase enzyme activities (SOD,T-AOC and GSH-PX),and highly significantly decrease MDA content,and significantly up-regulate gene expressions of SOD-1,SOD-2,GPX-1 andGPX-4  in type 1 diabetic mice.Therefore,vitexin can improve the antioxidant capacity of type 1 diabetic mice.

Key words: vitexin, type 1 diabetes mellitus, antioxidant, gene expression

中图分类号:  R965.1

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