This study aims to screen for antimicrobial Q-markers of Artemisia argyi Levl.et Vant.essential oil based on spectrum-effect relationships.The A. argyi essential oil was extracted by steam distillation method.The fingerprint of 15 batches samples was constructed by gas chromatography-mass spectrometer (GC-MS),and then the heat map and cluster analysis were performed.The antibacterial activities of different batches of A. argyi essential oil against Candida albicans,Escherichia coli and Staphylococcus aureus were detected.And then the Q-markers was screened by grey correlation analysis and monomer composition verification experiment.The results found that forty-nine chemical components were identified by GC-MS,and seventeen common peaks were screened out,including eucalyptol,(+)-2-bornanone,endo-borneol,etc,with the similarity between 0.96-0.99.The results of cluster analysis can be divided into two categories.The component with high abundance and greater differences in heat map display in the essential oil were selected as eucalyptol,(+)-2-bornanone,endo-borneol,and terpinen-4-ol.The inhibition zone of 15 batches of essential oil against C. albicans,E. coli and S. aureus was 9.00-11.56,9.33-12.44 and 9.33-12.89 mm,respectively.The results of grey correlation analysis showed that eucalyptol,(+)-2-bornanone and endo-borneol were the potential Q-markers for the antibacterial activity of A. argyi essential oil.The verification test results also showed that the activity of other compounds was consistent with the results of grey correlation analysis except for eucalyptol.In conclusion,(+)-2-bornanone and endo-borneol were the main Q-markers of A. argyi essential oil against bacteria.