天然产物研究与开发 ›› 2026, Vol. 38 ›› Issue (5): 1007-1016.doi: 10.16333/j.1001-6880.2026.5.009 cstr: 32307.14.1001-6880.2026.5.009

• 开发研究 • 上一篇    下一篇

基于Box-Behnken响应面法的杜仲叶中总黄酮纯化工艺研究

董小丽1,2，谢兴文3，李瑞婷1,2，张家旭1,2，马趣环2，石晓峰1,2*   

  1. 1甘肃中医药大学，兰州 730000；2甘肃省医学科学研究院，兰州 730050；3甘肃中医药大学附属医院，兰州 730000
  • 出版日期:2026-05-27 发布日期:2026-05-26
  • 基金资助:
    甘肃省科技重大专项（22ZD6FA021-4）；兰州市青年科技人才创新项目（2025-QN-116）

Purification process of total flavonoids from Eucommia ulmoides leaves based on Box-Behnken response surface method

DONG Xiao-li1,2,XIE Xing-wen3,LI Rui-ting1,2,ZHANG Jia-xu1,2,MA Qu-huan2,SHI Xiao-feng1,2*   

  1. 1Gansu University of Chinese Medicine,Lanzhou 730000,China;2Gansu Academy of Medical Sciences,Lanzhou 730050,China;3Affiliated Hospital of Gansu University of Chinese Medicine,Lanzhou 730000,Gansu,China
  • Online:2026-05-27 Published:2026-05-26

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摘要： 以杜仲叶为原料，研究大孔树脂分离纯化杜仲叶总黄酮的工艺。通过静态吸附解吸附及动态吸附试验筛选大孔树脂类型。并采用单因素试验考察该树脂对总黄酮纯化工艺的影响。在此基础上，以兼顾吸附率、解吸率及回收率的综合评分为评价指标，并设计响应面试验确定最佳工艺条件。考察的12种大孔吸附树脂中AB-8型树脂对杜仲叶总黄酮吸附分离性能良好。最佳工艺为：上样浓度2.78 mg/mL，上样体积3.55 BV，除杂溶剂为4 BV水，洗脱剂为75%乙醇，洗脱流速2 BV/h。在该条件下综合评分为73.60%，总黄酮纯度提高4.11倍，优化后的工艺简便、可行，为杜仲叶中总黄酮的进一步研究提供了实验依据。

关键词: 杜仲叶, 总黄酮, 分离纯化, 大孔树脂, 响应面法

Abstract:

This study investigated the separation and purification of total flavonoids from Eucommia ulmoides leaves using macroporous resins.Static and dynamic adsorption tests were used for resin screening,and single-factor experiments were performed to analyze parameter effects on purification efficiency.A comprehensive index considering adsorption,desorption,and recovery rates was adopted for evaluation,and response surface experiments were designed to optimize process conditions.In the 12 tested resins,AB-8 resin showed the best performance for flavonoid adsorption and separation.The optimal parameters were confirmed as:sample loading concentration 2.78 mg/mL,loading volume 3.55 BV,impurity removal with 4 BV ultrapure water,elution with 75% ethanol at a flow rate of 2 BV/h.Under these conditions,the comprehensive score reached 73.60%,and the purity of total flavonoids was improved 4.11 times.This simple,practical optimized process provides a basis for further research on Eucommia ulmoides leaves flavonoids.

Key words: Eucommia ulmoides leaves, total flavonoids, separation and purification, macroporous resin, response surface methodology

中图分类号:  R284.2

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