响应面法优化深层发酵樟芝总三萜的提取工艺（英文）

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (5): 946-951.

响应面法优化深层发酵樟芝总三萜的提取工艺（英文）

陆震鸣1，龚劲松2，何喆1，许泓瑜1，窦文芳1，史劲松3，许正宏1,2*

1江南大学医药学院制药工程研究室；2江南大学工业生物技术教育部重点实验室；3江南大学医药学院生物活性物质加工工程研究室，无锡 214122

收稿日期:2010-10-12 出版日期:2011-10-25 发布日期:2011-09-26

Optimization of Extraction of Total Triterpenoids from Submergedly Cultured Antrodia camphorata using Response Surface Methodology

LU Zhen-ming1, GONG Jin-song2, HE Zhe1, XU Hong-yu1, DOU Wen-fang1, SHI Jin-song3, XU Zheng-hong1,2*

1Laboratory of Pharmaceutical Engineering,School of Medicine and Pharmaceutics,Jiangnan University; 2The Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University; 3Laboratory of Bioactive Products Process Engineering,School of Medicine and Pharmaceutics,Jiangnan University,Wuxi 214122,China

Received:2010-10-12 Online:2011-10-25 Published:2011-09-26
Supported by:
This work was supported by the grants from Natural Science Foundation of Jiangsu Province,China (No.BK2010142),National High-Tech Program of China (No.2007AA021506),and the program for New Century Excellent Talents in University of China (No.NCET-07-0380).

摘要/Abstract

摘要： 采用响应面法对樟芝深层培养中总三萜的提取工艺进行优化。根据Box-Benhnken的中心组合实验设计原理，在单因素实验的基础上，选取溶剂浓度、提取温度和液固比为变量，应用响应面法进行三因素三水平的实验设计。以总三萜得率作为响应值，建立了樟芝总三萜提取的回归模型，对其提取条件进行进一步优化。结果表明，优化的总三萜提取条件为乙醇浓度86%，提取温度75 ℃，液固比37。进一步的实验也验证了该模型的有效性。

关键词: 樟芝, 深层培养, 三萜, 响应面法, 提取工艺优化

Abstract: The optimum conditions for the extraction of total triterpenoids from submergedly cultured Antrodia camphorata were determined by using response surface methodology (RSM).A three-level Box-Behnken factorial design was used to investigate the effects of three independent variables,namely solvent composition,extraction temperature and liquid-solid ratio on the response,the yield of total triterpenoids.The independent variables were coded at three levels and their actual values were selected on the basis of preliminary experimental results.Results showed that the generated regression models adequately explained the data variation and significantly represented the actual relationship between the independent variables and responses.A solvent composition of 86%,an extraction temperature of 75 ℃ and a liquid-solid ratio of 37 were found to be optimal for triterpenoid extraction from A.camphorata in submerged culture.By means of additional experiments,the adequacy of this model was conformed.

Key words: Antrodia camphorata, submerged culture, triterpenoid, response surface methodology, optimization of extraction

中图分类号:

R284.2

陆震鸣，龚劲松，何喆，许泓瑜，窦文芳，史劲松，许正宏. 响应面法优化深层发酵樟芝总三萜的提取工艺（英文）[J]. 天然产物研究与开发, 2011, 23(5): 946-951.

LU Zhen-ming,G ONG Jin-song, HE Zhe, XU Hong-yu, DOU Wen-fang,S HI Jin-song, XU Zheng-hong. Optimization of Extraction of Total Triterpenoids from Submergedly Cultured Antrodia camphorata using Response Surface Methodology[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(5): 946-951.

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