乌贼皮胶原蛋白的提取及结构表征

doi:A

摘要/Abstract

摘要： 为了充分利用乌贼加工废弃物，分析了乌贼皮的基本组成成分，优化了从乌贼皮中提取胶原蛋白的工艺条件，并利用SDS-PAGE垂直电泳、紫外扫描和傅里叶变换红外光谱对所提取的胶原蛋白进行了结构表征。结果表明，乌贼皮中含有大量胶原蛋白，可作为胶原蛋白来源的补充。采用酸酶复合提取胶原蛋白的最佳条件为：酒石酸浓度为0.1 mol/L，胃蛋白酶添加量为1400 U/g，料液比为1:20 (m:V，原料)，4 ℃提取18 h，提取率为12.08%。SDS-PAGE垂直电泳、紫外扫描和傅里叶变换红外光谱的结果表明，采用酸酶复合法从乌贼皮中提取的胶原蛋白为Ⅰ型胶原蛋白，保持了完整的三螺旋结构。

关键词: 胶原蛋白, 乌贼皮, 提取, 结构表征

Abstract: In order to take full advantage of cuttlefish (Sepia esculenta) processing waste, the basic components of cuttlefish skin were analyzed and the extraction conditions for collagen from cuttlefish skin were optimized. Along with this, purified collagen was analyzed through SDS-PAGE vertical electrophoresis, UV scanning and FT-IR. The results showed that cuttlefish skin contained large amounts of collagen and it could be used as the source of complement for collagen. The optimum conditions for collagen with acid and pepsin were determined as follows: soaking cuttlefish skin in 0.1 mol/L tartaric acid solution with a ratio of material to liquid of 1:20 (m:V, raw materials) at 4 ℃ for 18 h, the 1400 U/g pepsin was added in the beginning for the restriction hydrolysis, and the extraction yield of collagen was 12.08%. SDS-PAGE vertical electrophoresis, UV scanning and FT-IR results indicated that the extracted collagen was typeⅠ collagen and the unique triple helical structure of typeⅠcollagen remained well.

Key words: collagen, cuttlefish skin, extraction, structure characterization

中图分类号:

TS254.9 Q58

雷静，李和生，张丽媛，孙楠楠. 乌贼皮胶原蛋白的提取及结构表征[J]. 天然产物研究与开发, 2012, 24(5): 575-580.

LEI Jing, LI He-sheng, ZHANG Li-yuan, SUN Nan-nan. Extraction and Characterization of Collagen from Skin of Sepia esculenta[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 24(5): 575-580.

参考文献 1

Hao ZL(郝振林),Zhang XM(张秀梅),Zhang PD(张沛东).Biological characteristics and multiplication techniques of Sepia esculenta.Chin J Ecology(生态学杂志),2007,26:601-606.2 Jiang DT(蒋挺大),Zhang CP(张春萍).The Collagen(胶原蛋白).Beijing:Chemical Industry Press,2001,1-60.3 Zhang JZ(张建忠).Research on preparation and characterization of collagen from skin of grass carp.Nanjing:Nanjing Agricultural University(南京农业大学),PhD,2007.4 Takeshi N,Eiji Y,Kei T,et al.Isolation and characterisation of collagen from the outer skin waste material of cuttlefish (Sepia lycidas).Food Chem,2001,72:425-429.5 Mario Hiram UM,Joe Luis AM,Maribel PJ,et al.Jumbo squid (Dosidicus gigas) mantle collagen:Extraction,characterization,and potential application in the preparation of chitosan–collagen biofilms.Bioresour Technol,2010,101:4212–4219.6 Zhang JZ(张建忠),An XX(安辛欣),Wang L(王林)et al.Isolation and Characterization of Acid-soluble and Pepsin-soluble Collagens from Skin of Grass Carp(Ctenopharyngodon idellus).Food Sci(食品科学),2006,12:556-558.7 Yan MY(闫鸣艳).Study on the structure and physical properties of collagen from walleye pollock (Theragra chalcogramma) skin.Qingdao:Ocean University of China(中国海洋大学),PhD,2009.8 Zhao YH(赵玉红),Gao T(高天).Characteristics of Collagen from Deer Bone.Food Sci(食品科学),2008,7:43-47.9 Zhong ZH(钟朝辉),Li CM(李春美),Gu HF(顾海峰) et al.Extraction and Basic Properties of Pepsin-soluble Collagen from Grass Carp (Ctenopharyndogon idellus) Scales.Fisheries Sci (水产科学),2007,2:91-95.10 Yi JB(易继兵),Li BF(李八方),Zhao X(赵雪).Extraction and the physical and chemical characterization of collagen in snail fish.Sci Tech Food Ind(食品工业科技),2010,3:65-69.11 Yang XQ(杨贤庆),Zhang S(张帅),Hao SX(郝淑贤)et al.Optimization of Extraction and Physicochemical Properties of Tilapia Skin Collagen.Food Sci(食品科学),2009,16:106-111.12 Duan R(段蕊),Zhang JJ(张俊杰),Jin YJRY(今野久仁彦)et al.Characteristics Comparison of Pepsin-soluble Collagen from Skin and Bone of Carp (Cyprinus Carpio).Food Ferm Ind(食品与发酵工业),2008,5:10-13. 13 Zhang H(张虹),Zhuo SZ(卓素珍),Dai ZY(戴志远).Extraction and Characterization of Collagen from Anglerfish（Lophius Litulon）Skin.J Chin Inst food Sci Technol(中国食品学报),2009,9(6):34-41.14 Liu LL(刘丽莉),Ma MH(马美湖),Yang XL(杨协力).Extraction and Characterization of TypeⅠ Collagen from Bovine Bone.Food Sci(食品科学),2010,2:87-92.15 Yan MY,Li BF,Zhao X,et al.Characterization of acid-soluble collagen from the skin of walleye pollock (Theragra chalcogramma).Food Chem,2008,107:1581-1586.16 Wang L,An XX,Yang FM,et al.Isolation and characterisation of collagen from the skin,scale and bone of deep-sea red fish(Sebastes mentella).Food Chem,2008,108:616-623.17 Muyonga JH,Cole CGB,Duodu KG .Fourier transform infrared (FTIR) spectroscopic study of acid soluble collagen and gelatin from skins and bones of young and adult Nile perch (Lates niloticus).Food Chem,2004,86:325-332.

[1]	陈忠艺, 朱安明, 杨秀霞, 段函妤, 刘俊希, 肖丰坤, 曲丽萍. 青刺果油粕中性均一多糖的结构表征及其抗炎活性研究[J]. 天然产物研究与开发, 2026, 38(1): 37-45.
[2]	黄滢, 崔龄, 汪建青, 宫钰蓉, 张文涵, 杨光耀, 张文根. 箬叶竹总黄酮提取工艺优化研究[J]. 天然产物研究与开发, 2025, 37(增刊1): 116-122.
[3]	王莉茹, 孙梓玮, 李玮, 崔伟亮, 孙爱军, 任英旭, 李慧芬. 体外仿生消化对生丹参、酒丹参总酚酸提取物成分及生物活性的影响[J]. 天然产物研究与开发, 2025, 37(9): 1658-1670.
[4]	刘思美, 周婷, 王曙, 陈怡, 叶本贵. 川贝母提取物对慢性阻塞性肺病大鼠炎症反应及氧化应激的影响[J]. 天然产物研究与开发, 2025, 37(9): 1724-1732.
[5]	王参政, 孙永芳, 周晔, 任昆, 王冬梅, 潘英妮, 任舒蒙, 刘晓秋. 温莪术多糖提取工艺优化、结构表征及对功能性便秘的改善作用研究[J]. 天然产物研究与开发, 2025, 37(7): 1195-1207.
[6]	肖付才, 邢晓轲, 李颖奎, 黄斐. 响应面法优化超声辅助提取沙棘叶多酚工艺及其抗氧化和降脂活性研究[J]. 天然产物研究与开发, 2025, 37(7): 1331-1340.
[7]	王蓉, 王俊龙, 李杭霏, 李紫薇. 山药叶多糖的磷酸化修饰、结构表征及体外抗氧化与降糖活性研究[J]. 天然产物研究与开发, 2025, 37(5): 807-815.
[8]	孙永进, 王泓杰, 李海凤, 胡江如, 蔡锦源, 赵云龙. 微波辅助提取法对西番莲果皮多糖理化性质和功能活性的影响[J]. 天然产物研究与开发, 2025, 37(5): 895-903.
[9]	王贞香, 李万年, 张进举, 魏心怀, 罗旭, 安琼. 离子液体辅助超声提取苦参多酚及其体外降血糖活性研究[J]. 天然产物研究与开发, 2025, 37(4): 636-646.
[10]	余璐, 董静茹, 王兰兰, 李好, 戴雪梅, 郭新勇, 李静, 张丽. 基于UPLC-Q Exactive MS的准噶尔柳花化学成分分析及抗脂肪肝作用体外机制研究[J]. 天然产物研究与开发, 2025, 37(4): 647-658.
[11]	张春艳, 王益蕊, 周霏娅, 李素清. 黑树莓酚类物质提取工艺优化及抗氧化、抑菌活性研究[J]. 天然产物研究与开发, 2025, 37(3): 491-501.
[12]	杨益娜, 曾燚, 邵会艳, 李严朗, 席星雨, 胡江苗, 杨柳. 白及干品与鲜品中白及胶的结构表征及其免疫活性研究[J]. 天然产物研究与开发, 2025, 37(3): 502-511.
[13]	樊秦, 王雅琼, 赵彩霞, 张志瑞, 张宏美, 宋冰, 阎教科, 戚欢阳. 基于药效学和代谢组学研究当归补血汤不同提取物对溶血性贫血大鼠的作用[J]. 天然产物研究与开发, 2025, 37(2): 223-232.
[14]	文敏, 赵芮, 孙美记, 彭成, 刘娟, 熊亮. 川牛膝化学成分及其舒张子宫平滑肌活性研究[J]. 天然产物研究与开发, 2025, 37(2): 252-261.
[15]	周敏, 韩青, 李彩凤. 菊苣根多糖酶法辅助双水相提取工艺优化及其降脂活性研究[J]. 天然产物研究与开发, 2025, 37(2): 283-292.