天然产物研究与开发 ›› 2012, Vol. 24 ›› Issue (5): 594-598.doi: A

• 研究论文 • 上一篇    下一篇

不同产区太子参氨基酸含量测定及多元统计分析

安 坤1,2,何 静1,2,万忠民1,2,阳小成1*,焦 威2*   

  1. 1成都理工大学材料与化学化工学院,成都 610059;2中国科学院成都生物研究所,成都 610041
  • 收稿日期:2011-08-23 出版日期:2012-05-25 发布日期:2012-06-12
  • 基金资助:

    中科院西部之光博士项目(科发人教字〔2011〕1 号),国家自然科学基金(21002098),科技重大新药平台“中药质量系统评价方法、体系及技术标准平台”

Determination and Multivariate Statistical Analysis of Amino Acid in Pseudostellaria heterophylla from Different Producing Areas

AN Kun1,2, HE Jing1,2, WAN Zhong-Min1,2, YANG Xiao-Chen1*, JIAO Wei2*   

  1. 1College of Material Chemistry and Chemical Technology, Chengdu Univerity of Technology, Chengdu 610059, China; 2Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
  • Received:2011-08-23 Online:2012-05-25 Published:2012-06-12

摘要: 本文采用高速氨基酸自动分析仪对太子参四大种植主产区(安徽、江苏、福建、贵州)的栽培品及河南的野生品,共13个批次太子参药材的氨基酸组成和含量进行测定。结果显示氨基酸含量基本顺序为:精氨酸(Arg)> 谷氨酸(Glu)> 天冬氨酸(Asp)> 脯氨酸(Pro),其中安徽宣城产太子参样品(S2)的16种氨基酸总含量和7种人体必需氨基酸含量最高。以上述4种主要氨基酸作为指标,探讨了不同产区太子参药材的内在品质。采用聚类分析法对氨基酸含量进行多元统计分析,表明氨基酸含量分析可体现样品产区间和产区内的差异性,可区别野生品与栽培品。这些结果为发展太子参道地药材的质量标准控制提供了依据。

关键词: 太子参, 氨基酸, 聚类分析

Abstract: The contents and composition of amino acids in 13 bathes of Pseudostellaria heterpphylla (collected from Anhui, Jiangsu, Fujian, Guizou and Henan) were determined by HITACH IL8800 high speed amino acid analyzer. The results showed main sequence of amino acid content was arginine (Arg) > glutamic acid (Glu) > aspartic acid (Asp) > proline (Pro). The total contents of 16 amino acids and essential amino acids were highest in samples collected from xuancheng (Anhui province). In order to discuss the intrinsic quality of these samples, the Arg, Glu, Asp and Pro were analyzed and compared as the quality index. In addition, the multivariate statistical method was used to analyze the differences of samples from different origins and the differences between wild and cultivated samples. These results could be used as references for the quality standard control of P. heterpphylla.

Key words: Pseudostellaria heterophylla, amino acid, cluster analysis

中图分类号: 

R917 R927.2