活性污泥中Fe(Ⅲ)还原微生物的分离、鉴定及还原特性

doi:A

天然产物研究与开发 ›› 2012, Vol. 24 ›› Issue (5): 627-630.doi: A

活性污泥中Fe(Ⅲ)还原微生物的分离、鉴定及还原特性

任丽平^1,2*，张智¹，李柏林¹,谭洪²

¹重庆大学城市建设与环境工程学院，重庆 400030；²西华师范大学生命科学学院，南充 637000

收稿日期:2011-10-19 出版日期:2012-05-25 发布日期:2012-06-12
基金资助:
国家“十一五”科技重大专项——三峡库区城市污水处理厂功能提升与污泥处理处置技术研究与综合示范（2009ZX07315-002）

Isolation and Identification of Fe(Ⅲ) Reducing Bacteria from Activated Sludge and Their Reducing Characteristics

REN Li-ping^1,2*, ZHANG Zhi¹, LI Bo-Lin¹, TAN Hong²

¹College of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, China; ²College of Biolojical Sciences, China West Normal University, Nanchong 637000, China

Received:2011-10-19 Online:2012-05-25 Published:2012-06-12

摘要/Abstract

摘要： 采用平板分离法和柠檬酸铁还原实验法相结合，从城市污水处理厂活性污泥中分离获得Fe(Ⅲ)还原菌F7, 经形态观察、生理生化和16S rDNA序列分析及同源性比对鉴定为恶臭假单胞菌（Pseudomonas putida）。在不同柠檬酸铁浓度和不同pH条件下的实验表明，柠檬酸铁浓度为0.32 g/L时，菌株生长情况较好，柠檬酸铁浓度为0.16 g/L时，Fe(Ⅲ)异化还原比例较高；pH 6.5时，菌株生长情况较好，Fe(Ⅲ)异化还原量较多。

关键词: 活性污泥, Fe(Ⅲ)还原微生物, 系统发育分析, Fe(Ⅲ)异化还原

Abstract: A ferric reducing microorganism strain F7 was isolated from activated sludge taken from wastewater treatment plant through dilution-plate method and ferric citrate reduction. By means of the morphology, physiology and the 16S rDNA sequence analysis and homology comparison, strain F7 was identified as Pseudomonas putida. Different ferric citrate fluid medium concentration and different pH experiments showed that strain F7 growed well with 0.32 g/L ferric citrate, and Fe(Ⅲ) reduction ratio was high with 0.16 g/L ferric citrate, and the bacteria growth and Fe(Ⅲ) reduction quantity were well with pH 6.5.

Key words: activated sludge, ferric reducing microorganism, phylogenetic analysis, dissimilatory Fe(Ⅲ) reduction

中图分类号:

X172

任丽平，张智，李柏林,谭洪. 活性污泥中Fe(Ⅲ)还原微生物的分离、鉴定及还原特性[J]. 天然产物研究与开发, 2012, 24(5): 627-630.

REN Li-ping, ZHANG Zhi, LI Bo-Lin, TAN Hong. Isolation and Identification of Fe(Ⅲ) Reducing Bacteria from Activated Sludge and Their Reducing Characteristics[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 24(5): 627-630.

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活性污泥中Fe(Ⅲ)还原微生物的分离、鉴定及还原特性

Isolation and Identification of Fe(Ⅲ) Reducing Bacteria from Activated Sludge and Their Reducing Characteristics

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