黄花蒿青蒿酸制备及其生物稳定性研究

天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (10): 1557-1561.

黄花蒿青蒿酸制备及其生物稳定性研究

徐定华1,2，唐雯熙1,2，张晓蓉1,2*，李杰2，刘继旋2

1吉首大学植物资源保护与利用湖南省高校重点实验室；2吉首大学生物资源与环境科学学院，湖南吉首 416000

出版日期:2014-10-30 发布日期:2014-10-31

Preparation of Artemisinic Acid from Artemisia annua L.and Its Biological Stability

XU Ding-hua1,2, TANG Wen-xi1,2, ZHANG Xiao-rong1,2*, LI Jie2, LIU Ji-xuan2

1 Key Laboratory of Plant Resources Conservation and Utilization in Hunan Province,Jishou University; 2 College of Bioresources and Environment Science,Jishou University,Jishou 416000, China

Online:2014-10-30 Published:2014-10-31

摘要/Abstract

摘要： 基于青蒿酸重要的药用和开发价值，采用溶剂法、吸附分离法从黄花蒿植物制备青蒿酸，采用光谱、质谱和熔点法表征青蒿酸晶体，采用HPLC法分析其生物稳定性。通过制备工艺获得纯度为96%的青蒿酸结晶，产率为61.7%。4 ℃、室温、60 ℃以及自然光照条件下保存30 d青蒿酸具有较好的生物稳定性；植物体内外青蒿酸均能稳定保存一年，含量基本不变。紫外光照条件下青蒿酸易光解，6 h后基本检测不出青蒿酸。通过制备工艺获得高纯度的青蒿酸结晶，青蒿酸对温度和自然光照较为稳定，在植物体内外均可保存一年。紫外光对青蒿酸具有较强的破坏作用。

关键词: 黄花蒿, 青蒿酸, 制备工艺, 生物稳定性

Abstract: In this paper,the preparation of artemisinic acid from Artemisia annua L.was investigated and its biological stability was explored.Liquidliquid extraction method and adsorptive separation method were used to prepare artemisinic acid from A.annua,and spectroscopic,mass spectrometry and melting point method were adopted to characterize artemisinic acid crystals.The biological stability of artemisinic acid was analyzed using thin layer chromatography (TLC) and high performance liquid chromatography (HPLC).Artemisinic acid crystal was successfully obtained by liquidliquid extraction method and Adsorptive separation method,and the yield of product reached to 61.7% and the purity of product was higher than 96%.Artemisinic acid was very stable under the conditions of 4 oC,room temperature,60 ^oC and natural lighting for 30 d.Furthermore,it was stably preserved in plant for a year with its content maintained at the same level.The structure of product was badly destructed by UV light,and it was not detected after lighting for 6 h.In conclusion,high purity of artemisinic acid could be obtained by the developed preparation methods.It was more stable at the conditions of natural light and temperature,and the artemisinic acid could save one year in vivo and in vitro. However,UV light strongly destroy artemisinic acid.

Key words: Artemisia annua L., artemisinic acid;preparation technology;biological stability

中图分类号:

R284.6

徐定华，唐雯熙，张晓蓉，李杰，刘继旋. 黄花蒿青蒿酸制备及其生物稳定性研究[J]. 天然产物研究与开发, 2014, 26(10): 1557-1561.

XU Ding-hua1,2, TANG Wen-xi1,2, ZHANG Xiao-rong1,2*, LI Jie2, LIU Ji-xuan2. Preparation of Artemisinic Acid from Artemisia annua L.and Its Biological Stability[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(10): 1557-1561.

[1]	许嘉辉, 李鑫, 霍景琛, 崔春利, 张滢, 马裕霖, 张涓. 苦豆碱阴道用温敏凝胶的制备及其药效学研究[J]. 天然产物研究与开发, 2026, 38(1): 94-106.
[2]	李超, 刘云华, 秦登云, 黄志芳, 刘玉红, 陈燕, 易进海. 基于正交设计优化新鲜川芎中挥发油提取工艺[J]. 天然产物研究与开发, 2024, 36(5): 771-778.
[3]	薛昊#, 苏婧钰#, 陈文君, 申雨玫, 王可轩, 李鑫, 陈靖, 杨波. 双载体制备青蒿素速释型固体分散体工艺优化研究[J]. 天然产物研究与开发, 2021, 33(4): 620-629.
[4]	张邵博1,2，靳冬武3，李明生1,2*. 蛋白水解物制备工艺及其在生物技术领域中的应用研究进展[J]. 天然产物研究与开发, 2019, 31(2): 354-362.
[5]	丁刚1, 2, 3, 宋旭红1, 2, 3, 崔广林1, 2, 3, 谭均1, 2, 3, 李隆云1, 2, 3. UPLC-MS法同时测定青蒿7种有效成分的研究[J]. 天然产物研究与开发, 2018, 30(增刊2): 64-74.
[6]	王梦月，李雪梅，彭颖，彭崇胜，李晓波*. 紫玉簪活性甾体皂苷的制备工艺研究[J]. 天然产物研究与开发, 2018, 30(5): 875-881.
[7]	赵雅萌1，马玉楠1，陈传娇1，程永现2，董鲜1，徐福荣1. 黄花蒿叶水提物对三七根际尖孢镰刀菌生长的抑制作用[J]. 天然产物研究与开发, 2018, 30(3): 373-378.
[8]	夏鹏飞1,2,3，马肖4，吴国泰1,5，李阳1,2,3，樊秦1,2,3，赵磊1,2,3*. 龙胆苦苷纳米乳的制备工艺及质量安全性评价[J]. 天然产物研究与开发, 2017, 29(11): 1824-1830.
[9]	朱双双，汤逸飞，阮川芬，章华伟*. 黄花蒿内生真菌SPS-02的鉴定和次生代谢产物研究[J]. 天然产物研究与开发, 2016, 28(4): 519-524.
[10]	余泽民，王江南，何达海，丁克毅*. 青蒿母液中二氢青蒿酸的提取工艺优化[J]. 天然产物研究与开发, 2016, 28(10): 1652-1656.
[11]	郁霄1，潘魏松1，田浩1,2，王剑文1*. 盐胁迫对黄花蒿生长及其挥发性成分的影响[J]. 天然产物研究与开发, 2015, 27(9): 1539-1543.
[12]	侯豹1，冯磊2，王旭2，刘艳玲1，蔡维维2，邱丽颖2*. 王不留行黄酮苷软膏剂的制备工艺研究[J]. 天然产物研究与开发, 2015, 27(11): 1853-1857.
[13]	王领1，李富恒1*，程华2，何聪芬2，董银卯2. 马齿苋提取物制备工艺、安全性及抗敏功效的研究[J]. 天然产物研究与开发, 2014, 26(4): 583-587.
[14]	张晓蓉，陈功锡，陈良，潘涛，雷玉茸，徐定华. 驱蚊抑菌绿色蚊香工艺研制及其效果评价[J]. 天然产物研究与开发, 2011, 23(5): 962-966.