关键词: 榅桲, AB-8大孔吸附树脂, 总多酚, 纯化, PTP1B抑制作用

Abstract: In this study,11 types of macroporous resins were screened for the purification of Cydonia oblonga Mill.polyphenols.The optimal resin was selected through static absorption and desorption experiments.Furthermore,its dynamic absorption features and influencing factors were analyzed.The results showed that the optimal conditions for purification were as follows:macroporous resin AB-8 had the best separation efficiency when the concentration of sample solution was 0.04 g/mL,the adsorption flow rate was 2 BV/h,the loading weight was 11 BV,removing impurities by 3 BV of distilled water and eluting the total polyphenols with 4 BV of 50% ethanol-water at flow rate of 2 BV/h.The concentration of total polyphenols of the product after the adsorption concentrating on AB-8 resin achieved 30.11 mg/g,which was 3 times of crude extract (9.55 mg/g).The PTP1B inhibition effect of polyphenol extracts before and after the purification process was also discussed.The results indicated that,the total polyphenols of C.oblonga  had strong inhibition effect on PTP1B with IC₅₀ value of 16.12 μg/mL after purification by AB-8 resin and the IC₅₀ value was 78.14 μg/mL before purification.

Key words: Cydonia oblonga Mill., macroporous resin, total polyphenols, purification, PTP1B