关键词: 葡萄干, 糖, 气相色谱-质谱联用, 高效液相色谱-蒸发光散射检测, 提取工艺

Abstract: The saccharides in 8 different varieties of raisin were qualitatively analyzed with GC-MS,and quantitatively analyzed by HPLC-ELSD using Prevail Carbohydrate ES column under gradient elution with water and acetonitrile. The parameters of extraction temperature,solvent and solid/liquid ratio on the extraction of saccharides in raisin were investigated with single factor test. Results showed that optimized parameters for extraction were that the concentration of ethanol was 50% (V/V),the extraction temperature was 80 ℃,and solid/liquid ratio was 1:8. Eight saccharides (fructose,glucose,mannose,sucrose,inositol,maltose,galactose,Dsorbitol) were detected by GC-MS. The contents of fructose,glucose and sucrose in different raisins were quantitatively analyzed by HPLC-ELSD. The calibration curves exhibited good linearity (R²＞0.9992),the recoveries ranged from 92% to 109%,and the relative standard deviation (RSD) was lower than 2%. The total content of saccharides in varieties of raisins,except for Black corinth,were greater than 690 mg/g. The major ingredient was fructose and glucose.

Key words: raisins, saccharide, GC-MS, HPLC-ELSD, extraction process