Abstract: A method used for the extraction of safflower corolla was screened.Plant total RNA extraction kit,traditional Trizol,improved Trizol were used for the extraction of safflower corolla.Gel electrophoresis,RT-PCR and ultraviolet spectroscopy were used for detection.And the method screened was used for the total RNA extraction of safflower callus.The total RNA extraction of safflower Corolla used improved Trizol obtainedclearly visible gel electrophoresis three bands,RT-PCR results visible gel electrophoresis with a clear,UV spectrophotometry meet the requirements,the other two methods do not meet the requirements.The improved Trizol can used for the extraction of safflower corolla,the obtained total RNA can be used for the following experiment of Molecular Biology.

Key words: safflower corolla, total RNA extraction, plant total RNA extraction kit, traditional Trizol, improved Trizol